• cell adhesion

    mechanotransduction

    morphogenesis

    the DeSimone Laboratory at the University of Virginia

  • We study the emergence of

    biological form...

    Our research interest is morphogenesis; we are working to uncover cellular and molecular mechanisms involved in generating the proper three-dimensional organization of embryos and tissues. We focus primarily on cellular movements required for gastrulation and neurulation using embryos from the amphibian Xenopus laevis.

  • KEY PUBLICATIONS

    broken image

    Cadherin adhesion, tissue tension, and noncanonical wnt signaling regulate fibronectin matrix organization

    Dzamba, B., Jakab, K.R., Marsden, M., Schwartz, M.A., and DeSimone, D.W.

    (2009) Developmental Cell 16, 421-432

    broken image

    A Mechanoresponsive cadherin-keratin complex directs polarized protrusive behavior and collective cell migration

    Weber, G.F., Bjerke, M.A., and DeSimone, D.W.

    (2012) Developmental Cell 22, 104-115

    broken image

    Mechanical and signaling roles for keratin intermediate filaments in the assembly and morphogenesis of Xenopus mesendoderm tissue at gastrulation

    Sonavane, P.R., Wang, C., Dzamba, B., Weber, G.F., Periasamy, P. and DeSimone D.W.

    (2017) Development 144, 4363-4376

    broken image

     

    Modeling the roles of cohesotaxis, cell-intercalation, and tissue geometry in collective cell migration of Xenopus mesendoderm

    Comlekoglu, T., Dzamba, B.J., Pacheco, G., Shook, D.R., Sego, T.J., Glazier, J.A., Peirce, S.M. and DeSimone, D.W.

    (2024) Biology Open 13

  • PEOPLE

    Present and Past

    broken image

    Ivy Foundation Pratt Distinguished Professor of Morphogenesis, Principal Investigator

    Doug has been interested in the problem of morphogenesis since grad school. As a doctoral student with Mel Spiegel, he described blastomere lineage-specific differences in cell surface protein expression in sea urchin embryos. While a postdoc with Richard Hynes at MIT, he and his colleagues provided the first molecular characterization of integrin receptors. Since coming to UVA his lab has used Xenopus embryos to investigate the roles of extracellular matrix proteins, integrins and cadherins in cell adhesion and migration. Doug's current focus is to uncover the roles mechanical forces play in adhesion-dependent cell-signaling, morphogenesis and cell-fate determination.

    broken image

    Senior Scientist

    Bette was first introduced to extracellular matrix as a high school student intern at Ciba-Geigy corporation where she spent her summer sectioning arthritic rabbit knees. In graduate school at the University of Wisconsin, and then as a postdoc at Shriners Hospital in Portland, Oregon, she became fascinated by the question of how cells assemble and interact with their extracellular matrix. She joined Doug’s lab in order to study the dynamics of the matrix during the complex morphogenetic cell and tissue movements of early embryogenesis.

    broken image

    Senior Scientist

    Dave is in interested in how morphogenic machines work. He spent several years in the Keller lab investigating how cell movements generate morphogenic shape changes and how groups of cells work together to generate the forces that drive these changes. In the DeSimone lab, he has taken this to the next (smaller-scale) level, and is working to understand the cell biology underlying the biomechanics of morphogenic machines.

    broken image

    Gustavo Pacheco

    Graduate Student

    As an undergrad at the University of Chicago, Gustavo was intrigued by the simple invasion assays he conducted on Lymphangioleiomyomatosis cells, a rare lung cancer cell that is only found in women. He further explored his interest in cell migration as a post-bac at the NIH, where he characterized epithelial cancer cell migration at the single-cell level in 3D culture systems. He was especially excited to join the UVA Medical Scientist Training Program for his MD/PhD to study molecular mechanisms underlying collective cell migration in the DeSimone lab. In Gustavo's free time, he enjoys exploring the restaurant scenes of Charlottesville, Richmond, and Washington DC. In fact, he was able to eat fancy meals for free while in college by taking pretty pictures of the food!

    broken image

    Tien Comlekoglu

    Graduate Student

    Tien took some time off from UVA medical school in 2021 to work in the DeSimone lab and get bitten by the research bug. In the time since, he successfully gained admission into the Medical Scientist Training Program at UVA and is now working toward a Ph.D. in Biomedical Engineering co-mentored by Drs. DeSimone and Peirce-Cottler. Tien is interested in computational modeling of cell movements using both agent based and finite element methods.

    broken image

    Benjamin Edwards

    Lab and Research Technician

    Ben started out in science 25 years ago as a lab technician in another department at the UVA School of Medicine. His early research experience evolved into an interest in plant sciences. Ben went on to work in various agricultural settings, eventually becoming more focused on environmentally efficient agronomy, particularly in the areas of aquaculture and aquaponics. After a 20-year break pursuing ambitious farming ventures, Ben decided to apply his aquaculture knowledge in a new direction. He now oversees the general care of the lab's frog colony and manages day-to-day responsibilities in the lab. Ben's current role allows him to straddle his previous experience in aquaculture management while re-engaging with the academic side of science.

    c. 1996

    Sampson

    Lab Mascot

    Sampson brings more than 7 years of squirrel chasing experience to the lab. His primary goal is to one day catch a squirrel. When not pursuing varmints, Sampson likes to unwind with the PI.

    c. 1996

    Lab Alums

    Predocs - Postdocs - Support Staff

    David Ransom

    Fanying Meng

    Joe Ramos

    Charles A. Whittaker

    Benjamin Hoffstrom

    Katherine Smith

    Na Jie

    Guofeng Xu

    Tania Rozario

    Maureen Bjerke

    Glen Hirsh

    Crystal Richardson

    Pooja Sonavane

    Sandra J. Kateeshock

    Margaret Bolton

    Shannon O'Neil

    -

    Mark Hens

    Thomas Lallier

    Dominique Alfandari

    Theresa Curtis

    Alban Gaultier

    Helene Cousin

    Mungo Marsden

    Lance Davidson

    Karoly Jakab

    Shuo Wei

    Gregory Weber

    Chong Wang

    -

    Susan Dalton

    Laura Bolling

    Anne Allison

    Phoebe Williams

    Fred Simon

  • OPPORTUNITIES

    broken image

    POSITIONS AVAILABLE

    Postdoctoral and Predoctoral Training

    Please check back here for announcements of open positions and training opportunities at the predoctoral and postdoctoral levels. Prospective students are encouraged to apply to our Biomedical Sciences Graduate Program (BIMS). Existing BIMS students are invited to check out the Cell and Developmental Biology (CDB) Program at UVA and to come by and talk to Doug about lab rotation opportunities.

    broken image

    POSTDOCTORAL RESEARCH ASSOCIATE

    Position Announcement

    The DeSimone laboratory seeks a highly-motivated Postdoctoral Research Associate interested in exploring the importance of mechanical forces in the patterning and morphogenesis of embryos. Our lab has a long-standing interest in mechanisms of cell adhesion and adhesion-dependent cell signaling, and the involvement of these processes in regulating cell and tissue movements, gene expression and cell fate decisions, using Xenopus as a model system. Our research utilizes multiple approaches including live cell imaging, gene-editing, biochemistry and cell biology, and biomechanics. We are looking for a skilled cell and developmental biologist with high enthusiasm for science who will contribute positively to the multidisciplinary, collaborative atmosphere fostered within the DeSimone lab and the Department of Cell Biology.

  • CONTACT

    Where we are and how to reach us.

    CONTACT

     

    mailing address:

    DeSimone laboratory

    Department of Cell Biology

    University of Virginia

    P.O. Box 800732, School of Medicine

    Charlottesville, VA 22908

     

    physical address:

    Pinn Hall, room 3229

    1340 Jefferson Park Avenue

     

    38o 1' 54.642" N

    78o 30' 1.9296" W

     

    lab phone:

    434-924-1881

     

     

    broken image